Isolation and Characterization of Novel Broad Host Range Bacteriophages of Vibrio cholerae O1 from Bengal

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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

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Sounak Sarkar1, Mayukh Das2, Tushar Suvra Bhowmick2

  • 1Division of Bacteriology, Vibrio Phage Reference Laboratory, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.

Journal of Global Infectious Diseases
|June 19, 2018
PubMed
Abstract

Insights

Five novel, broad-host-range cholera phages were identified to enhance Vibrio cholerae strain discrimination. These new phages offer improved phage typing capabilities for epidemiological studies.

Area of Science:

  • Microbiology
  • Bacteriology
  • Virology

Background:

  • Cholera remains a significant public health concern, necessitating accurate strain identification.
  • Existing phage typing schemes for Vibrio cholerae require continuous improvement to keep pace with evolving strains.

Purpose of the Study:

  • To isolate and characterize novel broad-host-range cholera phages.
  • To evaluate the utility of these new phages in an extended phage typing scheme for Vibrio cholerae.

Main Methods:

  • Isolation and characterization of five new cholera phages.
  • Electron microscopy for phage morphology determination.
  • Phage sensitivity testing against 300 Vibrio cholerae strains from India.

Main Results:

  • The newly isolated phages exhibited broad host range and differed from existing ones.
  • Electron microscopy revealed phages belonging to myophage and podophage groups.
  • 100% of Vibrio O1 strains were typeable with the five new phages, with 40% belonging to Type-1.

Conclusions:

  • The novel Vibrio phages are effective for precise strain discrimination.
  • Incorporating these phages into existing systems will enhance Vibrio cholerae phage typing.
  • These phages represent a valuable tool for cholera surveillance and research.
Keywords:
BacteriophagesVibrio choleraeisolationmorphologyphage typingplaque

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