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Updated: Feb 8, 2026
Chronic Salmonella Infected Mouse Model
Published on: May 31, 2010
Effects of 1,8-cineole on Carbohydrate Metabolism Related Cell Structure Changes of Salmonella
Yangying Sun1, Xiaojun Cai1, Jinxuan Cao1
1Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Zhejiang, China.
Abstract:
Salmonella is gram-negative foodborne zoonotic bacteria which triggers disease in humans. Our previous studies showed 1,8-cineole possessed remarkable antimicrobial effects on foodborne zoonotic bacteria indicating it could serve as a new source of antibiotic for Salmonella. Present study elucidated the antibacterial mechanism of 1,8-cineole by analyzing serum protein expressed by Salmonella following treatment with 1,8-cineole (0.25 mg/mL, 3 h) using isobaric tags for relative and absolute quantification (iTRAQ) with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS). 1,8-cineole was found to significantly damage the structure of Salmonella cell walls and membranes. A total of 3011 proteins were extracted from the experimental group, of which 435 were differentially expressed (1.5-fold) with 123 upregulated and 312 downregulated. The expressed proteins were involved in 935 intracellular biological processes, 98 cellular components, 477 molecular functions and 86 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Among them, proteins associated with carbohydrate, nucleotide, amino acid, lipid, and energy metabolism were significantly changed following treatment with 1,8-cineole. Carbohydrate metabolism and membrane protein-related genes was down-regulated at the mRNA level when Salmonella was treated with 1,8-cineole. 1,8-cineole may be a potential antibiotic for Salmonella infections.
Insights
1,8-cineole damages Salmonella cell structures and alters protein expression, indicating its potential as a novel antibiotic for treating Salmonella infections. This study reveals its antibacterial mechanism by analyzing Salmonella
Area of Science:
- Microbiology
- Bacteriology
- Proteomics
Background:
- Salmonella is a significant foodborne zoonotic bacterium causing human diseases.
- 1,8-cineole has demonstrated antimicrobial properties against foodborne zoonotic bacteria.
Purpose of the Study:
- To elucidate the antibacterial mechanism of 1,8-cineole against Salmonella.
- To identify protein expression changes in Salmonella following 1,8-cineole treatment.
Main Methods:
- Isobaric tags for relative and absolute quantification (iTRAQ) coupled with 2D-LC-MS/MS were used to analyze Salmonella serum proteins.
- Differential protein expression was quantified, with 123 proteins upregulated and 312 downregulated.
Main Results:
- 1,8-cineole treatment significantly damaged Salmonella cell walls and membranes.
- Proteomic analysis revealed differential expression of 435 proteins involved in metabolism, cellular components, and molecular functions.
- Key metabolic pathways, including carbohydrate and energy metabolism, were significantly altered.
Conclusions:
- 1,8-cineole exhibits potent antibacterial activity against Salmonella by disrupting cellular structures and altering protein expression.
- The findings suggest 1,8-cineole is a promising candidate for developing new antibiotics against Salmonella infections.
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