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Updated: Feb 8, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli
Nan Jiang1,2, Guo-Liang Ying3,4, Ali K Yetisen5
1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing , Wuhan University of Technology , 122 Luoshi Road , Wuhan , 430070 , China .
Abstract:
Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and l-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.
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