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Oral Bacterial Infection and Shedding in Drosophila melanogaster
09:32

Oral Bacterial Infection and Shedding in Drosophila melanogaster

Published on: May 31, 2018

12.5K

Oral Bacterial Infection and Shedding in Drosophila melanogaster

Jonathon A Siva-Jothy1, Arun Prakash1, Radhakrishnan B Vasanthakrishnan2

  • 1Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh.

Journal of Visualized Experiments : Jove
|June 19, 2018
PubMed

Insights

This study presents a robust protocol for orally infecting fruit flies (Drosophila melanogaster) with bacteria. This method aids in studying gut immunity and pathogen transmission, revealing increased susceptibility in specific mutant fly lines.

Area of Science:

  • * Invertebrate immunology
  • * Infectious disease modeling
  • * Drosophila melanogaster research

Background:

  • * The fruit fly, Drosophila melanogaster, is a key model for studying innate immunity.
  • * Research is shifting from systemic infections to understanding gut immunocompetence.
  • * Oral infection methods are crucial for studying gut-specific immune responses.

Purpose of the Study:

  • * To establish a reliable protocol for the oral infection of Drosophila melanogaster.
  • * To enable the study of gut immunocompetence against bacterial pathogens.
  • * To investigate factors influencing pathogen transmission via oral routes.

Main Methods:

  • * Oral exposure of individual flies to Pseudomonas aeruginosa and Pseudomonas entomophila.
  • * Assessment of survival rates, microbial loads, and bacterial shedding.
  • * Utilizing Dcy and Relish mutant fly lines to validate the infection model.

Main Results:

  • * Demonstrated survival phenotypes, microbe loads, and bacterial shedding patterns.
  • * Confirmed increased susceptibility to oral bacterial infection in Dcy and Relish mutants.
  • * Highlighted heterogeneity in pathogen transmission dynamics.

Conclusions:

  • * The developed protocol provides a robust method for oral fly infection.
  • * This approach facilitates research into genetic and environmental influences on gut infections.
  • * The model is valuable for studying bacterial transmission and host immune responses.

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