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Nucleotide sequence of mouse prolactin and growth hormone mRNAs and expression of these mRNAs during pregnancy
Abstract:
The mRNAs for mouse prolactin and growth hormone have been isolated from anterior pituitary glands and cloned as cDNAs. The nucleotide sequences of these mRNAs have been determined, and these sequences, along with the predicted amino acid sequences, are compared to those of other mammalian prolactin and growth hormone mRNAs. Levels of prolactin and growth hormone mRNAs during pregnancy have been monitored by hybridization to the cloned cDNA probes. We find the levels of these mRNAs to remain nearly constant during mid-to-late gestation.
Insights
Researchers isolated mouse prolactin and growth hormone mRNAs, comparing their sequences to other mammals. mRNA levels remained constant during mid-to-late pregnancy.
Area of Science:
- Molecular Biology
- Endocrinology
- Genetics
Background:
- Prolactin and growth hormone are key pituitary hormones regulating mammalian physiology.
- Understanding the molecular basis of hormone regulation is crucial for reproductive biology.
Purpose of the Study:
- To isolate and characterize mouse prolactin and growth hormone messenger RNAs (mRNAs).
- To compare the nucleotide and amino acid sequences of these mouse hormones with other mammalian counterparts.
- To investigate the expression levels of these mRNAs during pregnancy.
Main Methods:
- Isolation of mRNAs from mouse anterior pituitary glands.
- Cloning of mRNAs as complementary DNAs (cDNAs).
- Nucleotide sequencing and sequence comparison.
- Hybridization assays using cDNA probes to quantify mRNA levels.
Main Results:
- Successfully isolated and sequenced mouse prolactin and growth hormone mRNAs.
- Comparative analysis revealed similarities and differences with other mammalian hormone sequences.
- Quantification showed stable levels of prolactin and growth hormone mRNAs during mid-to-late gestation.
Conclusions:
- The study provides valuable sequence data for mouse prolactin and growth hormone.
- Hormone mRNA levels are maintained consistently during late pregnancy, suggesting stable regulatory mechanisms.
- These findings contribute to understanding pituitary hormone gene expression during gestation.