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Updated: Feb 8, 2026

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Neurodevelopmental Reflex Testing in Neonatal Rat Pups
Published on: April 24, 2017
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Acoustic Reflex Testing in Neonatal Hearing Screening and Subsequent Audiological Evaluation.
Lilian Cássia Bórnia Jacob-Corteletti1, Eliene Silva Araújo2, Josilene Luciene Duarte3
1Department of Audiology and Speech Pathology, Bauru Dental School, University of São Paulo, Brazil.
Summary
Neonates with lower birth weight and gestational age showed reduced acoustic reflex elicitation and higher thresholds, indicating a higher risk for hearing impairment. Broadband noise effectively elicited responses in all infants.
Area of Science:
- Neonatal audiology
- Otoacoustic emissions
- Hearing screening
Background:
- Neonatal hearing loss affects 1-3 per 1000 live births.
- Early detection and intervention are crucial for optimal language and cognitive development.
- Risk factors for neonatal hearing loss include low birth weight and prematurity.
Purpose of the Study:
- To evaluate acoustic reflex screening and thresholds in healthy neonates and those at risk.
- To determine the impact of birth weight and gestational age on acoustic stapedial reflex (ASR).
Main Methods:
- Assessed 18 healthy neonates and 16 at-risk neonates using transient evoked otoacoustic emissions (TEOAEs).
- Conducted acoustic reflex screening and threshold tests with pure-tone and broadband noise stimuli.
- Analyzed the influence of gestational age and birth weight on ASR.
Main Results:
- Lower gestational age and birth weight were associated with reduced ASR elicitation by pure tones, particularly at 0.5, 1, and 2 kHz.
- At-risk neonates (Group II) exhibited higher ASR thresholds and absent reflexes more frequently compared to healthy neonates (Group I).
- Broadband noise elicited responses in all neonates, while pure-tone stimuli revealed significant differences between groups at 1, 2, and 4 kHz.
Conclusions:
- Birth weight and gestational age significantly influence acoustic reflex elicitation in neonates.
- Infants with risk factors like low birth weight and prematurity are less likely to exhibit ASR and have elevated thresholds.
- ASR testing, especially with broadband noise, is valuable for neonatal hearing assessment, with pure-tone results indicating higher risk in preterm or low birth weight infants.
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