Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Freezing Point Depression and Boiling Point Elevation03:12

Freezing Point Depression and Boiling Point Elevation

40.3K
Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
40.3K
Sperm Transport01:15

Sperm Transport

3.6K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
3.6K
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

15.2K
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
15.2K
Ideal Solutions02:24

Ideal Solutions

22.6K
According to Raoult’s law, the partial vapor pressure of a solvent in a solution is equal or identical to the vapor pressure of the pure solvent multiplied by its mole fraction in the solution. However, Raoult's Law is only valid for ideal solutions. For a solution to be ideal, the solvent-solute interaction must be just as strong as a solvent-solvent or solute-solute interaction. This suggests that both the solute and the solvent would use the same amount of energy to escape to the...
22.6K
General Properties of Solutions02:12

General Properties of Solutions

36.0K
Many common substances around us exist as a solution, such as ocean water, air, and gasoline. All solutions are mixtures of substances that are composed of varying amounts of two or more types of atoms or molecules. A mixture with a non-uniform composition is a heterogeneous mixture, whereas a mixture with a uniform composition is a homogeneous mixture. The components that make the homogeneous mixture are evenly spread out and thoroughly mixed. 
36.0K
Solution Formation02:16

Solution Formation

37.9K
There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride.
This selective...
37.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paternal dietary macronutrients affect the seminal vesicle fluid proteome and fetal development: a geometric framework for nutrition study in mice.

Proceedings. Biological sciences·2026
Same author

Towards cervical AI of sheep with stored semen: latest insights and future developments.

The Journal of reproduction and development·2026
Same author

The current status of artificial reproductive technologies in sheep.

The Journal of reproduction and development·2026
Same author

Thigmotactic navigation and functional enrichment of boar sperm in microfluidic channels.

Reproduction (Cambridge, England)·2026
Same author

Fixative composition and sample storage conditions influence the proportion of morphologically normal bull sperm.

Reproductive biology·2026
Same author

Interactive Mixed Reality Simulation Enhances Student Knowledge and Ultrasound Interpretation in Sheep Pregnancy Diagnosis.

Veterinary sciences·2026

Related Experiment Video

Updated: Feb 8, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.2K

Sublethal sperm freezing damage: Manifestations and solutions.

Taylor Pini1, Tamara Leahy1, Simon P de Graaf1

  • 1School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, New South Wales, 2006, Australia.

Theriogenology
|June 19, 2018
PubMed
Summary
This summary is machine-generated.

Sublethal freezing damages sperm, reducing fertility. This review examines freezing damage, its effects on sperm function, and potential cryoprotective agents for improved semen cryopreservation.

Keywords:
CryopreservationDamageInjurySpermatozoaSublethal

More Related Videos

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

40.8K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

32.1K

Related Experiment Videos

Last Updated: Feb 8, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.2K
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

40.8K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

32.1K

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Animal Science

Background:

  • Semen cryopreservation is vital for breeding, conservation, and reproductive medicine.
  • Sublethal freezing damage significantly limits cryopreservation success, impacting fertility.

Purpose of the Study:

  • To review the multifaceted nature of sublethal freezing damage in sperm.
  • To explore the impact of this damage on sperm function and in vivo performance.
  • To investigate cryoprotective strategies involving seminal plasma and proteins.

Main Methods:

  • Literature review of studies on semen cryopreservation and freezing damage.
  • Analysis of research on sperm cryocapacitation, oxidative stress, and molecular alterations.
  • Examination of studies on sperm-female reproductive tract interactions and fertility outcomes.

Main Results:

  • Sublethal freezing induces cryocapacitation, reactive oxygen species, and molecular changes in sperm.
  • Damage affects sperm lifespan and interactions with the female reproductive tract.
  • Seminal plasma and specific proteins show cryoprotective potential.

Conclusions:

  • Understanding sublethal freezing damage is crucial for optimizing semen cryopreservation.
  • Further research into cryoprotective agents can enhance fertility outcomes.
  • Improved cryopreservation techniques benefit artificial breeding, conservation, and medicine.