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Updated: Feb 8, 2026

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
Published on: November 28, 2016
Hemocytes and granular cell fragments of Tipula paludosa larvae
1Biology Department, Liverpool Polytechnic, Byrom Street, Liverpool L3 3AF, UK.
Abstract:
Five morphological categories of hemocyte (prohemocyte, ameboid plasmatocyte, spindle-shaped plasmatocyte, oenocytoid, and granular cell) were observed by light and electron microscopy of hemolymph from Tipula paludosa larvae. In addition, vast numbers of membrane-bounded granule-containing fragments were present, and were also found in the larval hemolymph of four other Tipula spp. The fragments appear to be derived from the granular cells, which readily fragment. Granules in the granular cells and the fragments appear to have similar substructure. Melanization and coagulation of the hemolymph occur rapidly on exposure to air; the granular cell fragments may be concerned with one or both of these processes.
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