Related Experiment Video
Updated: Feb 8, 2026
12:52
Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
Published on: September 9, 2014
16.4K
Subcellular stoichiogenomics reveal cell evolution and electrostatic interaction mechanisms in cytoskeleton
Yu-Juan Zhang1,2, Chengxu Zhu1, Yiran Ding1
1Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Shapingba, Chongqing, 401331, People's Republic of China.
BMC Genomics
|June 20, 2018
Summary
Stoichiogenomics reveals distinct element compositions in cellular compartments. Eukaryotic cytoskeletal proteins evolved increased charged amino acids, facilitating electrostatic interactions for vital functions.
Area of Science:
- Cell Biology
- Biochemistry
- Bioinformatics
Background:
- Eukaryotic cells feature diverse subcellular compartments.
- Stoichiogenomics analyzes elemental composition in biomacromolecules.
- Subcellular stoichiogenomic adaptations remain largely unexplored.
Purpose of the Study:
- To define and apply stoichiogenomics to subcellular environments.
- To investigate elemental composition differences across cellular compartments.
- To elucidate the role of amino acid content in cytoskeletal protein function.
Main Methods:
- Updated the definition of stoichiogenomics.
- Applied stoichiogenomic analysis to subcellular proteomes.
- Analyzed amino acid content and electrostatic interactions in cytoskeletal proteins.
Main Results:
- Identified unique nitrogen content in nuclear proteomes and hydrogen/sulfur in extracellular proteomes.
- Found highest acidic amino acid content in cytoskeletal proteins.
- Observed increased electrostatic interactions in cytoskeletal primary sequences and 3D structures.
Conclusions:
- Established a framework for subcellular stoichiogenomic characteristics.
- Demonstrated evolutionary increase in charged amino acids within the cytoskeleton.
- Linked increased charged amino acids to electrostatic interactions, supporting cytoskeletal functions like activation and complex formation.
Related Concept Videos
02:55The Evidence for Evolution
48.3K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
48.3K
01:54Convergent Evolution
33.0K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
33.0K
01:18Polarity of the Cytoskeleton
25.3K
The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...
25.3K
01:16Electrostatic Boundary Conditions
974
Consider an external electric field propagating through a homogeneous medium. When the electric field crosses the surface boundary of the medium, it undergoes a discontinuity. The electric field can be resolved into normal and tangential components. The amount by which the field changes at any boundary is given by the difference between the field components above and below the surface boundary.
The surface integral of an electric field is given by Gauss's law in integral form and is related to...
The surface integral of an electric field is given by Gauss's law in integral form and is related to...
974
01:33Introduction to the Cytoskeleton
35.0K
Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶ microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶ microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
35.0K
01:17Studying the Cytoskeleton
9.7K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
9.7K