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Long non-coding RNA RUNXOR accelerates MDSC-mediated immunosuppression in lung cancer.

Xinyu Tian1,2, Jie Ma2, Ting Wang3

  • 1Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212012, China.

BMC Cancer
|June 20, 2018
PubMed
Summary
This summary is machine-generated.

Long non-coding RNA RUNXOR is upregulated in lung cancer patients and associated with myeloid-derived suppressor cells (MDSCs). RUNXOR may be a therapeutic target for anti-tumor therapy by modulating MDSC immunosuppression.

Keywords:
Anti-tumor immunityLung cancerMDSCsRUNX1lncRNA RUNXOR

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Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Long non-coding RNA RUNX1 overlapping RNA (RUNXOR) regulates myeloid cell development by targeting runt-related transcription factor 1 (RUNX1).
  • Myeloid-derived suppressor cells (MDSCs) are key immunosuppressive cells in cancer.
  • The role of RUNXOR in MDSC development was previously unknown.

Purpose of the Study:

  • To investigate the role of lncRNA RUNXOR in the development and function of MDSCs in lung cancer.
  • To determine the relationship between RUNXOR expression and MDSCs in lung cancer patients.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to measure RUNXOR and RUNX1 expression.
  • Isolation of human MDSCs from lung cancer patient tumor tissues via flow cytometry (FCM) or induction from peripheral blood mononuclear cells (PBMCs).
  • ব্যবহার of specific small interfering RNA (siRNA) to knockdown RUNXOR expression in MDSCs.

Main Results:

  • RUNXOR expression was significantly upregulated in the peripheral blood of lung cancer patients.
  • RUNX1 expression was downregulated in lung cancer patients, consistent with RUNXOR acting as a target gene.
  • RUNXOR expression was higher in MDSCs from tumor tissues compared to adjacent tissues.
  • Knockdown of RUNXOR in MDSCs led to decreased Arginase 1 (Arg1) expression.

Conclusions:

  • RUNXOR is significantly associated with MDSC-mediated immunosuppression in lung cancer.
  • RUNXOR may serve as a potential therapeutic target for anti-tumor strategies in lung cancer.