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Updated: Feb 8, 2026
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
Published on: December 13, 2012
36.5K
Multiplexed Division Tracking Dyes for Proliferation-Based Clonal Lineage Tracing
Miles B Horton1,2, Giulio Prevedello3, Julia M Marchingo1,2
1The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Victoria, Australia.
Journal of Immunology (Baltimore, Md. : 1950)
|June 20, 2018
Summary
Researchers developed a new high-throughput method to track cellular family trees and understand how cell diversity arises in immune responses. This technique aids in studying clonal contributions to cell fate selection.
Area of Science:
- Immunology
- Cell Biology
- Genetics
Background:
- Cellular heterogeneity is crucial for immune responses.
- Understanding clonal contributions to cell fate selection is challenging.
- Current methods for lineage tracing are laborious.
Purpose of the Study:
- To introduce a novel, high-throughput method for measuring familial fate changes.
- To provide statistical tools for hypothesis testing in lineage analysis.
- To enable detailed study of intraclonal and interclonal heterogeneity.
Main Methods:
- Combines multiplexing of division tracking dyes with phenotypic marker detection.
- Enables high-throughput measurement of clonal lineage properties.
- Applied to in vitro-activated mouse CD8+ T cell cultures.
Main Results:
- Successfully reported division and phenotypic changes at the family level in CD8+ T cells.
- Demonstrated the method's ability to reveal clonal lineage properties.
- Quantified familial fate changes with accompanying statistical analysis.
Conclusions:
- The novel method offers an accessible and high-throughput approach to study cellular heterogeneity.
- This technique has broad utility across various cell types and experimental systems (in vitro and potentially in vivo).
- Facilitates a deeper understanding of heritability and asymmetry in phenotypic changes during immune responses.
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