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Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
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A versatile nanobody-based toolkit to analyze retrograde transport from the cell surface

Dominik P Buser1, Kai D Schleicher2, Cristina Prescianotto-Baschong2

  • 1Biozentrum, University of Basel, CH-4056 Basel, Switzerland dominik-pascal.buser@unibas.ch martin.spiess@unibas.ch.

Proceedings of the National Academy of Sciences of the United States of America
|June 20, 2018
PubMed
Summary

Researchers developed functionalized nanobodies to track cell surface protein transport to the Golgi. This tool quantifies retrograde transport pathways, aiding in understanding cellular homeostasis and compartment identity.

Keywords:
AP-1mannose-6-phosphate receptorsnanobodiesretrograde transporttyrosine sulfation

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