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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
Published on: June 19, 2017
PyA-cluster system for the detection and imaging of miRNAs in living cells through double-three-way junction
Jong Jin Ro1, Ha Jung Lee1, Byeang Hyean Kim1
1Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea. bhkim@postech.ac.kr.
Abstract:
Herein, we describe an extended version of a fluorescence probe for detecting miRNAs through the novel application of a PyA-cluster system. By testing various (CG)n sequences in the middle of the oligonucleotide strand of the probe, we obtained an optimal sequence that formed a double-three-way-junction structure, with two PyA units positioned close together, in the presence of the target miRNA. This system readily detected the locations of target miRNAs in living cells and allowed visualization of structural changes through variations in the color of the fluorescence.
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