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Updated: Feb 8, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
Effects of Fluoride on Autophagy in Mouse Sertoli Cells
Zhiyuan Feng1, Chen Liang1, Ram Kumar Manthari1
1Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China.
Abstract:
Fluoride had been reported to damage the structure and function of testicular tissues and reproductive cells; however, the mechanisms underlying its toxicity remained unclear. Autophagy plays a key role in reproductive function. In this study, we aimed to investigate the effect of fluoride on autophagy in Sertoli cells. Sertoli cells were exposed to 0, 0.125, 0.25, and 0.5 mM NaF for 24 h. The results showed that fluoride exposure up-regulated Beclin1 and p62 mRNA and protein expression levels with concomitant down-regulated mRNA and protein expression levels of LC3 and Atg5. In conclusion, exposure to fluoride impaired the autophagy process in Sertoli cells, which could be one of fluoride's mechanisms in male reproductive toxicity.
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