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Updated: Feb 8, 2026

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
Published on: October 17, 2017
GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds
Tomoo Shimada1, Kentaro Fuji1, Takuji Ichino1
1Graduate School of Science, Kyoto University, Kyoto, Japan.
Abstract:
Vacuolar trafficking plays a vital role in plant growth and development. In this chapter, we describe a powerful technique for the evaluation of vacuolar protein trafficking, which is designated as GREEN FLUORESCENT SEED. Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.
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