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Related Concept Videos

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In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
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Related Experiment Video

Updated: Feb 8, 2026

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
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Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections.

Teresa Adell1, Sara Barberán2, Miquel Sureda-Gómez2

  • 1Department of Genetics, Microbiology, and Statistics, Institute of Biomedicine, University of Barcelona, Barcelona, Catalonia, Spain. tadellc@ub.edu.

Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2018
PubMed
Summary

We detail protocols for paraffin embedding and immunostaining of planarian flatworms. These methods are crucial for studying stem cell regeneration at the cellular level in Schmidtea mediterranea.

Keywords:
AntibodyImmunohistochemistryParaffin histological sectionsPlanarianSchmidtea mediterranea

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Area of Science:

  • Zoology
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Planarians are model organisms renowned for their remarkable regenerative capabilities.
  • Studying regeneration at the cellular level requires effective histological and molecular techniques.
  • Immunohistochemistry is a key method for visualizing cellular processes during regeneration.

Purpose of the Study:

  • To provide detailed protocols for paraffin embedding and immunostaining of planarian flatworms.
  • To establish a reliable method for cellular-level analysis of planarian regeneration.
  • To facilitate mechanistic studies in the model species Schmidtea mediterranea.

Main Methods:

  • Detailed step-by-step protocols for paraffin embedding of planarian specimens.
  • Immunostaining procedures for paraffin-embedded sections of Schmidtea mediterranea.
  • Validation of the protocol using various commercially available antibodies.

Main Results:

  • Robust and reproducible immunostaining results were achieved.
  • The protocol is effective for visualizing cellular details in planarian tissues.
  • The methods are adaptable for new antibodies and other planarian species.

Conclusions:

  • Established protocols for paraffin embedding and immunostaining are presented.
  • These methods are essential for advancing research in planarian stem cell biology and regeneration.
  • The provided procedures serve as a foundation for future mechanistic investigations.