Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Imaging Studies VII: Vascular Imaging01:19

Imaging Studies VII: Vascular Imaging

373
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
373
X-ray Imaging01:24

X-ray Imaging

10.5K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.5K
Brain Imaging01:14

Brain Imaging

754
Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
754
Imaging Studies IV: Magnetic Resonance Imaging01:27

Imaging Studies IV: Magnetic Resonance Imaging

283
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
283
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

9.7K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-edited safe and immune-evasive human pluripotent cells: Potential solution for allogeneic therapies.

Stem cell reports·2026
Same author

Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Biglycan fragment modulates TGF-β activity in intervertebral disc via an eIF6-coupled intracellular path.

Science advances·2025
Same author

Proteomic profiling of human plasma and intervertebral disc tissue reveals matrisomal, but not plasma, biomarkers of disc degeneration.

Arthritis research & therapy·2025
Same author

Effects of GDF6 on active protein synthesis by cells of degenerated intervertebral disc.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2025
Same author

An inducible mouse model of osteogenesis imperfecta type V reveals aberrant osteogenesis caused by Ifitm5 c.-14C>T mutation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2025

Related Experiment Video

Updated: Feb 8, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

9.9K

Live Imaging of Planaria.

Wei Shen1, Yun Shen2, Yun Wah Lam1

  • 1Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong.

Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2018
PubMed
Summary

This study introduces a novel agarose embedding method to immobilize planarians for extended time-lapse imaging. This technique overcomes previous challenges, enabling detailed observation of regeneration and physiological processes.

Keywords:
Fluorescent dye labelingLive imagingPlanarianRegenerationTime-lapse microscopy

More Related Videos

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
07:41

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

Published on: December 4, 2020

4.1K
Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

11.0K

Related Experiment Videos

Last Updated: Feb 8, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

9.9K
Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
07:41

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

Published on: December 4, 2020

4.1K
Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

11.0K

Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Animal Imaging

Background:

  • Planarian regeneration is a complex process requiring precise spatial and temporal cellular coordination.
  • Time-lapse imaging offers valuable insights into tissue dynamics in model organisms.
  • Previous time-lapse imaging of planarians was hindered by animal photophobia and delicate body structure.

Purpose of the Study:

  • To develop an effective method for immobilizing planarians for long-term time-lapse imaging.
  • To enable detailed observation of planarian regeneration and other physiological processes using live imaging.

Main Methods:

  • A new embedding technique utilizing 2% (w/v) low melting agarose was developed.
  • The method successfully immobilizes planarians for up to 7 days.
  • Integration with cell-permeable fluorescent dyes facilitates imaging.

Main Results:

  • The agarose embedding method provides stable immobilization of planarians.
  • This technique allows for continuous, high-resolution time-lapse imaging over extended periods.
  • Successful imaging of planarian regeneration and physiological activities was achieved.

Conclusions:

  • The described agarose embedding method is a significant advancement for planarian research.
  • This technique overcomes key challenges in planarian live imaging.
  • It opens new avenues for studying complex biological processes in planarians.