Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.3K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

15.0K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
15.0K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.6K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.4K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.2K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translation and cross-cultural adaptation of the Dubowitz Neurological Examination for premature infants in a high-risk outpatient clinic in Brazil.

Jornal de pediatria·2025
Same author

Homage to Michel Bornens, who passed away on March 9, 2022 at the age of 84.

EMBO reports·2024
Same author

Female Germline Cysts in Animals: Evolution and Function.

Results and problems in cell differentiation·2023
Same author

Evolution: The ancient history of cilia assembly regulation.

Current biology : CB·2023
Same author

Transgrediens Form of Pachydermodactyly.

Journal of cutaneous medicine and surgery·2023
Same author

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis.

The EMBO journal·2022

Related Experiment Video

Updated: Feb 8, 2026

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
09:20

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Published on: October 14, 2011

14.1K

Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques.

John L Brubacher1, Ana P Vieira2, Juliette Azimzadeh3

  • 1Canadian Mennonite University, Winnipeg, MB, Canada. jbrubacher@cmu.ca.

Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2018
PubMed
Summary

Optimized protocols for Schmidtea mediterranea (S. mediterranea) prepare the flatworm for ultrastructural analysis. These methods are crucial for studying regeneration and development at cellular and subcellular levels.

Keywords:
Histological methodsMicrowave tissue processingPlanariansSpecimen handling methodsTransmission electron microscopyUltrastructural methods

More Related Videos

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.3K
Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy
08:30

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Published on: July 15, 2019

10.7K

Related Experiment Videos

Last Updated: Feb 8, 2026

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
09:20

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Published on: October 14, 2011

14.1K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.3K
Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy
08:30

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Published on: July 15, 2019

10.7K

Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Zoology

Background:

  • Schmidtea mediterranea is a key model organism for stem cell biology, regeneration, and evolutionary studies.
  • Existing molecular tools for S. mediterranea are advanced, but ultrastructural techniques require optimization.
  • Understanding histological, cellular, and subcellular features is vital for regeneration and development research.

Purpose of the Study:

  • To present optimized protocols for preparing Schmidtea mediterranea for ultrastructural analysis.
  • To provide reliable tissue-processing methods for freshwater planarians.

Main Methods:

  • Detailed protocols for standard chemical fixation.
  • A rapid microwave-based fixation technique.

Main Results:

  • Optimized protocols suitable for S. mediterranea tissue preparation.
  • Demonstration of effective standard chemical fixation.
  • Demonstration of effective rapid microwave-based fixation.

Conclusions:

  • The presented protocols enhance the utility of S. mediterranea as a model system for ultrastructural studies.
  • These methods will aid in a deeper understanding of regeneration and development in planarians.