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CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Updated: Feb 8, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes.

Katia Tarasava^1,2, Eun Joong Oh², Carrie A Eckert^2,3

¹Chemical and Biological Engineering, University of Colorado, Boulder, CO, USA.

Biotechnology Journal

|June 20, 2018

Summary

This summary is machine-generated.

CRISPR-Cas technologies are revolutionizing microbial engineering with advanced genome editing and non-editing tools. These innovations enhance high-throughput genome-scale engineering in model organisms and enable new applications for biotechnology.

Keywords:

CRISPR editing CRISPR gene regulation CRISPR tools for microbes CRISPRa CRISPRi

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Area of Science:

Microbial biotechnology and synthetic biology
Molecular biology and genome engineering

Background:

CRISPR-Cas technologies have significantly advanced microbial engineering capabilities.
Existing tools enable genome editing and non-editing applications, expanding engineering scope and scale.
The need for versatile tools is growing as biotechnological applications expand to diverse organisms.

Purpose of the Study:

To summarize current advances in CRISPR-Cas based microbial gene editing.
To highlight state-of-the-art methods for high-throughput genome engineering in model organisms.
To review non-editing CRISPR-Cas applications for gene expression, epigenetics, RNA editing, and synthetic circuits.

Main Methods:

Review of recent literature on CRISPR-Cas systems in microbial engineering.
Focus on genome editing and non-editing applications.
Emphasis on high-throughput and genome-scale engineering strategies in *Escherichia coli* and *Saccharomyces cerevisiae*.

Main Results:

CRISPR-Cas tools have expanded the throughput and scale of microbial engineering.
Methods for efficient genome-scale engineering in *E. coli* and *S. cerevisiae* are highlighted.
Diverse non-editing applications including gene expression, epigenetic remodeling, RNA editing, and synthetic gene circuits are reviewed.

Conclusions:

CRISPR-Cas technologies offer powerful and versatile tools for microbial engineering.
Further research is needed to expand the utility and range of applications for these methods.
Continued development will enhance genome manipulation capabilities in both model and non-model organisms.