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Adenovirus sequences required for replication in vivo.

K Wang, G D Pearson

Nucleic Acids Research

|July 25, 1985

Summary

Adenovirus DNA replication origins were mapped to the terminal repeat sequences. A minimal origin and an auxiliary region, containing nuclear factor I binding sites, significantly enhance adenovirus replication efficiency.

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Area of Science:

Molecular Biology
Virology
Genetics

Background:

Adenoviruses are DNA viruses that replicate in host cell nuclei.
Understanding viral DNA replication mechanisms is crucial for developing antiviral strategies.
The terminal sequences of adenovirus DNA are known to play a role in replication.

Purpose of the Study:

To investigate the in vivo replication properties of plasmids with deletions in adenovirus terminal sequences.
To precisely map the DNA replication origin of adenovirus.
To identify functional domains within the replication origin and their contribution to replication efficiency.

Main Methods:

Deletion mapping of cloned adenovirus terminal sequences in plasmids.
Analysis of in vivo replication properties of modified plasmids.
Identification of functional domains and regulatory elements within the origin of replication.

Main Results:

The adenovirus DNA replication origin was localized to the first 67 base pairs of the inverted terminal repeat.
Two functional domains were identified: a minimal origin (18-21 bp) and an auxiliary region.
The auxiliary region, containing nuclear factor I binding sites, increased replication efficiency over 100-fold.

Conclusions:

The minimal origin contains conserved sequences essential for adenovirus replication initiation.
The auxiliary region significantly enhances replication efficiency through interactions with host factors like nuclear factor I.
Precise mapping of the origin and its regulatory elements provides insights into adenovirus DNA replication control.

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