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Related Experiment Videos

Human N-ras: cDNA cloning and gene structure.

A Hall, R Brown

    Nucleic Acids Research
    |July 25, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers elucidated the human N-ras gene structure by analyzing cDNA clones, revealing seven exons and two mRNA transcripts. This study details the gene

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Gene Expression

    Background:

    • Understanding the structure of oncogenes like N-ras is crucial for cancer research.
    • Previous knowledge on the specific organization and transcriptional variants of the human N-ras gene was limited.

    Purpose of the Study:

    • To determine the detailed structure and organization of the human N-ras gene.
    • To characterize the two main mRNA transcripts originating from the N-ras gene.
    • To investigate the transcriptional start sites and promoter region of the N-ras gene.

    Main Methods:

    • Analysis of cDNA clones derived from human N-ras mRNA.
    • DNA sequencing of cDNA clones to determine gene structure.
    • S1 nuclease protection assays to map transcriptional start sites.

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  • Bioinformatic analysis of promoter regions and untranslated sequences.

    • Main Results:

    • The human N-ras gene comprises seven exons.
    • Two distinct mRNA transcripts (4.3 Kb and 2 Kb) were identified, differing in their termination site.
    • Two closely spaced transcriptional start sites were mapped.
    • The promoter region lacks a TATA box but contains GGGCGG repeats; the 5' UTR has two upstream ATGs.

    Conclusions:

    • The study provides a comprehensive structural map of the human N-ras gene.
    • The findings clarify the molecular basis for alternative N-ras mRNA transcripts.
    • The identified promoter elements offer insights into N-ras gene regulation.