Can nano-SiO₂ reduce the phytotoxicity of acetaminophen? - A physiological, biochemical and molecular approach

This study aimed at evaluating the interactive effects of acetaminophen (AC; 400 mg kg^-1) and silicon dioxide nanomaterial (nano-SiO₂;3 mg kg^-1) on soil-grown barley. After 14 days of growth, plant growth, evaluated in terms of fresh and dry weight, was greatly inhibited by AC, independently of being or not co-treated with nano-SiO₂. Plants growing under high levels of AC did not show any increase in malondialdehyde (MDA) nor thiols contents, though levels of superoxide anion (O₂^.-) and hydrogen peroxide (H₂O₂) were increased in leaves and roots, respectively. When plants were co-treated with nano-SiO₂, reactive oxygen species (ROS) content remained unchanged, but lipid peroxidation (LP) was diminished and the thiol redox network was up-regulated in roots. The evaluation of the response of the antioxidant system showed that AC affected both non-enzymatic and enzymatic components in an organ-specific manner: proline levels and superoxide dismutase (SOD) activity were enhanced, whilst catalase (CAT) activity decreased in leaves; ascorbate content and CAT activity were diminished in roots. In response to the nano-SiO₂ co-treatment, this pattern was not vastly altered, despite for ascorbate peroxidase (APX), whose activity was greatly enhanced in both organs. Overall, combining biometric, biochemical and molecular approaches, this study revealed that, although AC impaired plant growth and development, it did not trigger a harsh oxidative stress condition. Maybe by this reason, the ameliorating potential of nano-SiO₂ was not so evident; yet, nano-SiO₂ was able to reduce LP and to stimulate thiol content and APX activity, possibly as a defense mechanism against AC-induced stress.