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Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

57.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.2K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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5.5K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

15.5K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Updated: Feb 8, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

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RNA methylation in nuclear pre-mRNA processing.

Helena Covelo-Molares1, Marek Bartosovic1, Stepanka Vanacova1

  • 1CEITEC, Masaryk University, Brno, Czech Republic.

Wiley Interdisciplinary Reviews. RNA
|June 20, 2018
PubMed
Summary
This summary is machine-generated.

Eukaryotic messenger RNA (mRNA) modifications, particularly N6-methyladenosine (m6A), regulate gene expression. Recent research reveals these methylation marks are crucial for nuclear RNA processing and function.

Keywords:
RNA demethylaseRNA methylaseRNA processing

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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
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Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • Eukaryotic RNA modifications are diverse, with early focus on rRNA and tRNA.
  • Recent advances enable study of messenger RNA (mRNA) modifications, especially methylation.
  • N6-methyladenosine (m6A) is the most abundant mRNA modification, with more being discovered.

Purpose of the Study:

  • To review recent advances in RNA methylation research.
  • To highlight the role of methylation in nuclear RNA processing.
  • To understand methylation-dependent RNA processing in the nucleus.

Main Methods:

  • Transcriptome-wide studies
  • Advanced biochemistry
  • Discovery of methylation writers, readers, and erasers

Main Results:

  • mRNA methylation regulates diverse cellular, developmental, and disease processes.
  • Methylation impacts mRNA stability and translation.
  • Emerging evidence shows methylation regulates nuclear RNA processes.

Conclusions:

  • RNA methylation is a key regulator of eukaryotic gene expression.
  • Methylation plays significant roles in nuclear RNA processing.
  • Continued research is expanding the understanding of mRNA modifications and their functions.