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Phage Genetic Engineering Using CRISPR⁻Cas Systems.

Asma Hatoum-Aslan1

  • 1Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA. ahatoum@ua.edu.

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|June 21, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas systems offer powerful genetic engineering tools for bacteriophages (phages), enabling functional studies of these abundant viruses. This review details methods for phage genetic engineering using CRISPR-Cas, advancing virology research.

Keywords:
CRISPR–Casbacteriophagegenome editingphage genetics

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Virology

Background:

  • CRISPR-Cas systems are prokaryotic immune mechanisms.
  • Bacteriophages (phages) are the most abundant biological entities.
  • Many phage genes have unknown functions, necessitating genetic tools.

Purpose of the Study:

  • To review CRISPR-Cas systems for genetic engineering of phages.
  • To detail mechanisms, strategies, and efficacies of phage editing.
  • To highlight the potential of CRISPR-Cas for advancing phage biology.

Main Methods:

  • Review of CRISPR-Cas systems (Types I, II, III) for phage engineering.
  • Analysis of specific strategies and reported efficacies.
  • Synthesis of current literature on CRISPR-Cas mediated phage modification.

Main Results:

  • CRISPR-Cas systems can be harnessed to genetically engineer diverse phages.
  • Specific CRISPR-Cas types (I, II, III) have been successfully applied.
  • Phage editing efficacies vary depending on the system and host.

Conclusions:

  • CRISPR-Cas systems provide versatile tools for phage genetic engineering.
  • These tools will advance the mechanistic understanding of prokaryotic viruses.
  • CRISPR-Cas applications will accelerate novel phage-based technologies.