Related Experiment Video

Updated: Feb 8, 2026

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

7.2K

Notch signaling pathway mediates Doxorubicin-driven apoptosis in cancers

Zixin Huang1, Shuibin Lin2, Chongde Long1

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Cancer Management and Research
|June 21, 2018
PubMed
Abstract

Insights

Doxorubicin chemotherapy activates the Notch pathway, specifically HES1, which is crucial for inducing cancer cell death. Modulating this pathway may enhance Doxorubicin

Area of Science:

  • Oncology
  • Molecular Biology
  • Cellular Signaling

Background:

  • Doxorubicin is a vital chemotherapy agent with significant side effects.
  • Understanding Doxorubicin's molecular targets is key to improving cancer treatment efficacy.
  • The Notch signaling pathway's role in Doxorubicin's action requires elucidation.

Purpose of the Study:

  • To investigate the downstream molecular pathways of Doxorubicin in cancer cells.
  • To determine the role of the Notch signaling pathway in Doxorubicin-induced apoptosis.
  • To explore HES1 as a potential mediator of Doxorubicin's anti-cancer effects.

Main Methods:

  • HeLa S3 cells were treated with Doxorubicin.
  • Notch pathway gene expression was quantified using Real-Time qRT-PCR.
  • HES1 expression was manipulated via lentiviral transfection.
  • Cell proliferation and apoptosis were assessed using MTT assays and flow cytometry.
  • Protein localization was analyzed by immunofluorescence.

Main Results:

  • Doxorubicin significantly upregulated Notch pathway components in cancer cells.
  • Doxorubicin treatment activated the Notch target HES1, which is essential for apoptosis.
  • Overexpression of HES1 potentiated Doxorubicin-induced apoptosis.
  • HES1 mediated apoptosis by activating PARP1 and regulating AIF subcellular localization.

Conclusions:

  • Doxorubicin's anti-cancer effects involve the activation of the Notch signaling pathway.
  • HES1 plays a critical role in mediating Doxorubicin-induced cancer cell apoptosis.
  • Targeting the Notch pathway could enhance Doxorubicin's efficacy in cancer therapy.
Keywords:
DoxorubicinHES1NotchPARP1apoptosiscancer treatment

More Related Videos

Stimulation of Notch Signaling in Mouse Osteoclast Precursors08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.4K
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

951

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.5K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.7K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K