Change in VO2max and time trial performance in response to high-intensity interval training prescribed using ventilatory threshold
Related Experiment Video
Updated: Feb 8, 2026

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Todd A Astorino1, Jamie deRevere2,3, Theodore Anderson2,4
1Department of Kinesiology, California State University, San Marcos, 333. S. Twin Oaks Valley Road, UNIV 320, San Marcos, CA, 92096-0001, USA. astorino@csusm.edu.
Abstract:
Completion of high-intensity interval training (HIIT) leads to significant increases in maximal oxygen uptake (VO2max) and oxidative capacity. However, individual responses to HIIT have been identified as approximately 20-40% of individuals show no change in VO2max, which may be due to the relatively homogeneous approach to implementing HIIT.
Purpose:
This study tested the effects of HIIT prescribed using ventilatory threshold (VT) on changes in VO2max and cycling performance.
Methods:
Fourteen active men and women (age and VO2max = 27 ± 8 year and 38 ± 4 mL/kg/min) underwent nine sessions of HIIT, and 14 additional men and women (age and VO2max = 22 ± 3 year and 40 ± 5 mL/kg/min) served as controls. Training was performed on a cycle ergometer at a work rate equal to 130%VT and consisted of eight to ten 1 min bouts interspersed with 75 s of recovery. At baseline and post-testing, they completed progressive cycling to exhaustion to determine VO2max, and on a separate day, a 5 mile cycling time trial.
Results:
Compared to the control group, HIIT led to significant increases in VO2max (6%, p = 0.007), cycling performance (2.5%, p = 0.003), and absolute VT (9 W, p = 0.005). However, only 57% of participants revealed meaningful increases in VO2max and cycling performance in response to training, and two showed no change in either outcome.
Conclusions:
A greater volume of HIIT may be needed to maximize the training response for all individuals.
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