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Updated: Feb 8, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

microRNA-30d mediated breast cancer invasion, migration, and EMT by targeting KLF11 and activating STAT3 pathway

Mingli Han1,2, Yimeng Wang3, Guangcheng Guo1

  • 1Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Journal of Cellular Biochemistry
|June 21, 2018
PubMed

Insights

MicroRNA-30d (miR-30d) promotes breast cancer growth and metastasis by regulating the KLF-11-STAT3 pathway. Inhibiting miR-30d reverses these effects, suggesting miR-30d as a potential biomarker and therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-30d (miR-30d) is implicated in cancer development and shows potential as a breast cancer biomarker and therapeutic target.
  • The precise role and molecular mechanisms of miR-30d in breast cancer progression, including cell growth and metastasis, remain largely undefined.

Purpose of the Study:

  • To elucidate the role of the miR-30d-KLF-11-STAT3 pathway in breast cancer.
  • To investigate the impact of miR-30d on breast cancer cell proliferation, apoptosis, migration, invasion, and epithelial-mesenchymal transition (EMT).

Main Methods:

  • Quantitative analysis of miR-30d expression in breast cancer cell lines (BT474, MDA-MB-231, HCC197, MDA-MB-468) and a non-tumorigenic cell line (MCF10A).
  • Assessment of cell survival, apoptosis (Bcl-2, Bax expression), migration, and invasion following miR-30d mimic or inhibitor treatment.
  • Investigation of the direct targeting of KLF-11 by miR-30d and its effect on KLF-11 and pSTAT3 expression.

Main Results:

  • miR-30d levels were significantly elevated in multiple breast cancer cell lines compared to MCF10A.
  • miR-30d mimics enhanced cell survival, inhibited apoptosis, and promoted migration and invasion, mediating EMT.
  • miR-30d directly targets KLF-11, influencing KLF-11 and pSTAT3 levels, with inhibitors reversing these effects.

Conclusions:

  • miR-30d plays a critical role in breast cancer cell biology, dependent on KLF-11 and pSTAT3 levels.
  • miR-30d functions as a potential diagnostic biomarker and therapeutic target for breast cancer.
Keywords:
EMTKLF-11breast cancermetastasismiR-30dpSTAT3

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