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Updated: Feb 8, 2026
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Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
Published on: February 10, 2023
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Classification of genera of Pasteurellaceae using conserved predicted protein sequences
Henrik Christensen1, Magne Bisgaard2
11Department of Veterinary Animal Sciences, University of Copenhagen, 4 Stigbøjlen, DK-1870 Frederiksberg C, Denmark.
Summary
This study analyzed Pasteurellaceae bacterial phylogeny using whole genome sequences and 31 conserved proteins. Results confirm existing classifications but reveal misclassifications within Actinobacillus, Pasteurella, and Haemophilus genera.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- The Pasteurellaceae family is a diverse group of Gram-negative bacteria.
- Accurate phylogenetic classification is crucial for understanding bacterial evolution and relationships.
Purpose of the Study:
- To investigate the phylogeny of Pasteurellaceae species using whole genome sequences.
- To evaluate the current classification of genera within the Pasteurellaceae family.
Main Methods:
- Downloaded whole genome sequences for 49 type strains and three genomospecies.
- Selected 31 conserved proteins from predicted proteomes.
- Performed phylogenetic analysis using maximum-likelihood and neighbor-joining methods on concatenated protein sequences.
Main Results:
- Phylogenetic analysis confirmed existing genus classifications based on 16S rRNA gene sequences.
- Identified potential reclassifications for several species within Actinobacillus, Pasteurella, and Haemophilus.
- Observed high inter- and intra-genus pairwise similarity (88%) based on concatenated protein sequences.
Conclusions:
- The study validates current Pasteurellaceae classification but highlights the need for taxonomic revision in certain genera.
- Genomic and proteomic analyses provide robust insights into bacterial phylogeny.
- Further taxonomic studies are warranted for genera like Actinobacillus, Pasteurella, and Haemophilus.
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