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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
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German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
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Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
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Imaging Studies IV: Magnetic Resonance Imaging01:27

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Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
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The cell is chemically composed of water, organic molecules and inorganic ions.
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Related Experiment Video

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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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Multispectral Live-Cell Imaging.

Sarah Cohen1,2, Alex M Valm1,3, Jennifer Lippincott-Schwartz1,4

  • 1Eunice Kennedy Shriver National Center for Child Health and Human Development, NIH, Bethesda, Maryland.

Current Protocols in Cell Biology
|June 21, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new method for simultaneously imaging six different fluorescent reporters in live cells, overcoming spectral overlap limitations. This advanced live-cell imaging enables detailed analysis of cellular dynamics and organization.

Keywords:
fluorescent proteinsimaging informaticslinear unmixingorganellesspectral imaging

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Fluorescent proteins and vital dyes are essential for observing cellular processes.
  • Spectral overlap between fluorophores limits the number of reporters detectable simultaneously in live cells.

Purpose of the Study:

  • To develop and present a protocol for simultaneous imaging of six fluorophores in live cells.
  • To overcome the limitations imposed by spectral overlap in multicolor live-cell imaging.

Main Methods:

  • Utilizing a confocal microscope equipped with a spectral detector.
  • Applying linear unmixing algorithms to differentiate spectrally overlapping fluorophores.
  • Imaging live cells labeled with six distinct fluorescent probes.

Main Results:

  • Successful simultaneous imaging of six different fluorophores in live cells.
  • Demonstrated visualization of dynamics in six distinct organelles.
  • Enabled quantification of organelle contacts using multispectral imaging.

Conclusions:

  • Multispectral live-cell imaging offers a powerful approach for systems-level analysis of cellular organization and dynamics.
  • The presented protocol expands the capacity for multicolor imaging in live-cell studies.
  • This method is adaptable for visualizing any molecule tagged with a fluorescent probe.