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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
Published on: November 26, 2018
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Isolation of cells from whole blood using shear-induced diffusion
Jian Zhou1,2,3, Chunlong Tu4,5, Yitao Liang4,5
1Biosensor National Special Laboratory, Key Laboratory of BME of the Ministry of Education, Zhejiang University, Hangzhou, 310027, China. zhouja@zju.edu.cn.
Scientific Reports
|June 22, 2018
Summary
This study introduces a novel microfluidic platform for continuous cell separation from whole blood. The system uses blood
Area of Science:
- Biomedical Engineering
- Microfluidics
- Cell Separation
Background:
- Directly extracting cells of interest from whole blood is challenging due to complex blood properties.
- Existing methods often require complex sample processing or specialized reagents.
Purpose of the Study:
- To develop a microfluidic platform for continuous, size-selective cell separation from unprocessed whole blood.
- To leverage intrinsic blood properties for efficient cell focusing and isolation.
Main Methods:
- A novel microfluidic device utilizing shear-induced diffusion and fluid inertia.
- Employing saline solution to create a sandwiched fluid configuration.
- Continuous separation of beads and cells from whole blood.
Main Results:
- Achieved high separation efficiency of 89.8% for cells from whole blood.
- Demonstrated a high throughput of 6.75 mL/hr (10^6-10^7 cells/s).
- Successfully isolated circulating tumor cells (CTCs) from hepatocarcinoma patient blood samples.
Conclusions:
- The developed microfluidic platform offers an efficient method for continuous cell separation from whole blood.
- The technology shows promise for rapid isolation of biomarkers like CTCs for clinical applications.
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