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Measuring RAN Peptide Toxicity in C. elegans
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Glycoreplica Peptides.

Teruhiko Matsubara1, Toshinori Sato2, Takao Taki3

  • 1Department of Biosciences and Informatics, Keio University, Kouhoku, Yokohama, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|June 22, 2018
PubMed
Summary

Glycoreplica peptides mimic carbohydrate antigens using phage display technology. These peptides show potential as alternatives for carbohydrate-based vaccines and therapeutics for related diseases.

Keywords:
Anti-carbohydrate antibodyGlycosidase inhibitorLectinPhage display technologyRandom peptide library

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Area of Science:

  • Biochemistry and Molecular Biology
  • Immunology
  • Drug Discovery

Background:

  • Carbohydrate antigens play crucial roles in biological processes and diseases.
  • Developing alternatives to natural carbohydrate ligands is challenging.
  • Phage display technology offers a powerful tool for peptide and protein discovery.

Purpose of the Study:

  • To describe a method for identifying glycoreplica peptides using phage display.
  • To explore the potential of glycoreplica peptides as functional mimics of carbohydrate antigens.
  • To highlight their applications in vaccination and therapeutics.

Main Methods:

  • Utilizing a phage display peptide library.
  • Employing monoclonal antibodies recognizing carbohydrate epitopes.
  • Performing phage display selection against carbohydrate-binding proteins.
  • Identifying amino acid sequences of glycoreplica peptides.

Main Results:

  • Successfully prepared glycoreplica peptides that mimic carbohydrate antigen structures.
  • Demonstrated that these peptides can replicate functions of original glycoconjugate antigens.
  • Established a method for selecting target-specific peptides via phage display.

Conclusions:

  • Glycoreplica peptides can effectively mimic carbohydrate epitopes and their functions.
  • Phage display is a viable method for identifying these mimetic peptides.
  • These peptides hold significant promise as novel carbohydrate-based vaccines and therapeutics.