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Applications Of NMR In Biology01:25

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
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Recent Progress in Light Sheet Microscopy for Biological Applications.

Krishnendu Chatterjee1,2,3, Feby Wijaya Pratiwi1,2,4, Frances Camille M Wu2

  • 11 Nanoscience and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.

Applied Spectroscopy
|June 22, 2018
PubMed
Summary
This summary is machine-generated.

Light sheet fluorescence microscopy (LSFM) offers high resolution, speed, and minimal photodamage, advancing biomedical research. This review details LSFM

Keywords:
Light sheet microscopycellular imagingoptical imagingsuper-resolution microscopy

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Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Cell Biology

Background:

  • Conventional optical microscopy faces limitations in resolution, speed, and phototoxicity.
  • Light Sheet Fluorescence Microscopy (LSFM) has emerged as a significant advancement.
  • LSFM offers high 3D spatial resolution, signal-to-noise ratio, and rapid acquisition.

Purpose of the Study:

  • To provide a systematic review of Light Sheet Fluorescence Microscopy (LSFM).
  • To discuss historical development, variants, improvements, and technological advancements of LSFM.
  • To explore LSFM's potential applications across various biological research fields.

Main Methods:

  • Systematic literature review of Light Sheet Fluorescence Microscopy (LSFM).
  • Analysis of historical development and technological evolution of LSFM.
  • Compilation of LSFM's current and potential applications in biological research.

Main Results:

  • LSFM provides high 3D resolution, high signal-to-noise ratio, and fast imaging.
  • LSFM exhibits minimal phototoxicity and photodamage, crucial for live samples.
  • LSFM has diverse applications from single-cell dynamics to whole-organism imaging.

Conclusions:

  • LSFM overcomes limitations of conventional microscopy, offering superior imaging capabilities.
  • LSFM's unique properties enable advanced research in cell biology, developmental biology, and neuroscience.
  • Continued advancements in LSFM promise further breakthroughs in biological and clinical research.