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Updated: Feb 8, 2026

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
Stabilization of an intermolecular i-motif by lipid modification of cytosine-oligodeoxynucleotides
Yawei Sun1, Yanyun Ji, Dong Wang
1State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum (Huadong), Qingdao, 258000, China. yawsun@upc.edu.cn.
Abstract:
This paper describes the stabilization of an intermolecular i-motif by lipophilic modification on the 3'-terminus of oligonucleotides. The hydrophobic aliphatic chain connected at the 3'-terminus of a trinucleotide (dC)3 promoted the formation of an i-motif and significantly enhanced the quadruplex's stability. The impact of lipophilic modification on i-motif's thermal stability was studied by UV-thermal denaturation melting experiments and isothermal titration calorimetry. We found that alkyl chains containing more than 14 carbon atoms could elevate the i-motif structure's stability in a wide range of pH and concentrations.
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