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Updated: Feb 8, 2026
Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Synthesis of ENA Nucleotides and ENA Oligonucleotides
1Modality Research Laboratories, Daiichi Sankyo Co., Ltd, Shinagawa, Tokyo, Japan.
Abstract:
2'-O,4'-C-Ethylene-bridged nucleic acid (ENA) is a sugar-modified oligonucleotide with an ethylene bridge between the 2'-oxygen and 4'-carbon of ribose. ENA not only has as high binding affinity to complementary RNA as conventional bridged/locked nucleic acid, but also has much higher nuclease resistance in plasma, which makes it a promising candidate for antisense therapeutics. This unit presents detailed protocols for the synthesis and characterization of ENA nucleosides and oligonucleotides. © 2018 by John Wiley & Sons, Inc.
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