Related Experiment Video
Updated: Feb 8, 2026

Determination of Total Lipid and Lipid Classes in Marine Samples
Published on: December 11, 2021
Free lipid and computerized determination of adipocyte size
Henrik Svensson1, Daniel Olausson1, Agneta Holmäng2
1a Department of Clinical Chemistry and Transfusion Medicine , Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden.
Abstract:
The size distribution of adipocytes in a suspension, after collagenase digestion of adipose tissue, can be determined by computerized image analysis. Free lipid, forming droplets, in such suspensions implicates a bias since droplets present in the images may be identified as adipocytes. This problem is not always adjusted for and some reports state that distinguishing droplets and cells is a considerable problem. In addition, if the droplets originate mainly from rupture of large adipocytes, as often described, this will also bias size analysis. We here confirm that our ordinary manual means of distinguishing droplets and adipocytes in the images ensure correct and rapid identification before exclusion of the droplets. Further, in our suspensions, prepared with focus on gentle handling of tissue and cells, we find no association between the amount of free lipid and mean adipocyte size or proportion of large adipocytes.
More Related Videos
Related Concept Videos
Dose Size and Dosing Frequency: Determination Methods
What are Lipids?
What are Lipids?
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
Lipid Digestion
Structure of Lipids
Cell Size
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...

