Cellular targets of antilymphocyte antibodies in AIDS and LAS

Many patients with acquired immune deficiency syndrome (AIDS) and lymphadenopathy syndrome (LAS) have serum antilymphocyte antibodies. The targets of these antibodies are neither sex nor HLA directed. There is disagreement about the cell subset at risk. We examined the cellular specificity of antilymphocyte antibodies from AIDS and LAS patients by microcytotoxicity on positively selected lymphocytes, by using double-labeling immunofluorescence, and by using well-characterized continuous cell lines as targets. We find that most AIDS/LAS patients have antibodies to T and B cells, that immunofluorescence is somewhat more sensitive than microcytotoxicity, and that the antibodies are directed to both T4+ and T4- lymphoid cells.