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Mechanisms of antimelanoma effect of oat β-glucan supported by electroporation

Anna Choromanska1, Sandra Lubinska2, Anna Szewczyk3

  • 1Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.

Bioelectrochemistry (Amsterdam, Netherlands)
|June 22, 2018
PubMed

Insights

Oat beta-glucan (OβG) enters cells via Glucose Transporter Type 1 (GLUT1), a mechanism crucial for its anti-tumor effects. Electroporation enhances this OβG transport into cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The cellular uptake mechanisms for beta-glucan molecules remain largely uncharacterized.
  • Tumor cells exhibit higher uptake of glucose polymers (glucans) compared to normal cells, potentially due to transporter overexpression.
  • Increased Glucose Transporter Type 1 (GLUT1) expression is observed in malignant melanomas, correlating with cell growth and mitotic activity.

Purpose of the Study:

  • To investigate whether oat beta-glucan (OβG) is transported into cells via the GLUT1 membrane protein.
  • To determine if electroporation (EP) can enhance OβG cellular uptake.

Main Methods:

  • GLUT1 transporters were inhibited using the WZB117 inhibitor.
  • Cell viability was assessed in the presence and absence of the GLUT1 inhibitor.
  • The effect of reversible electroporation (EP) on OβG uptake was evaluated.

Main Results:

  • The study demonstrated that OβG uptake is dependent on GLUT1.
  • Inhibition of GLUT1 significantly affected OβG transport into cells.
  • Electroporation was found to enhance the cellular uptake of OβG.

Conclusions:

  • The transport of oat beta-glucan (OβG) into cells is mediated by the GLUT1 transporter.
  • Electroporation serves as a method to augment OβG delivery into cells.
  • These findings elucidate a key mechanism for OβG cellular uptake and its potential therapeutic applications.
Keywords:
GLUT1MelanomaOat beta-glucanWZB117

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