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Response to Comment on "Sterilizing immunity in the lung relies on targeting fungal apoptosis-like programmed cell

Neta Shlezinger1, Henriette Irmer2, Sourabh Dhingra3

  • 1Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10075, USA.

Science (New York, N.Y.)
|June 23, 2018
PubMed

Insights

This study investigates programmed cell death with apoptosis-like features (A-PCD) in Aspergillus fumigatus. We used multiple markers and fungal genes to accurately monitor fungal cell death and its role in infections.

Area of Science:

  • Mycology
  • Cell Biology
  • Immunology

Background:

  • Programmed cell death with apoptosis-like features (A-PCD) plays a role in fungal pathogenesis.
  • Accurate monitoring of fungal A-PCD is crucial for understanding host-pathogen interactions.

Purpose of the Study:

  • To address concerns regarding the interpretation of current assays for monitoring A-PCD in Aspergillus fumigatus.
  • To investigate the role of fungal A-PCD in host immune surveillance and infectious outcomes.

Main Methods:

  • Utilized multiple independent A-PCD markers to validate findings.
  • Employed genetic manipulations using fungal orthologs, avoiding reliance on mammalian counterparts.
  • Focused on Aspergillus fumigatus, a significant fungal pathogen.

Main Results:

  • The study provides a robust approach to monitoring fungal A-PCD.
  • Demonstrates the utility of fungal-specific genetic strategies in studying cell death pathways.
  • Highlights the importance of A-PCD in the context of fungal infections.

Conclusions:

  • The developed methodology offers a reliable way to study fungal A-PCD.
  • Findings contribute to a better understanding of how Aspergillus fumigatus interacts with the host immune system.
  • This research facilitates further studies into antifungal strategies targeting cell death pathways.

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