Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.9K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.9K
Thermal expansion and Thermal stress: Problem Solving01:27

Thermal expansion and Thermal stress: Problem Solving

2.2K
San Francisco's Golden Gate Bridge is exposed to temperatures ranging from -15 °C to 40 °C. At its coldest, the main span of the bridge is 1275 m long. Assuming that the bridge is made entirely of steel, what is the change in its length between these temperatures?
To solve the problem, first, identify the known and unknown quantities. The initial length (L) of the bridge is 1275 m, the coefficient of linear expansion (α) for steel is 12 x 10-6/°C, and the change in temperature (ΔT) is 55...
2.2K
Thermal Strain01:19

Thermal Strain

2.9K
Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.9K
Thermal Expansion01:22

Thermal Expansion

5.7K
The expansion of alcohol in a thermometer is one of many commonly encountered examples of thermal expansion, which is the change in size or volume of a given system as its temperature changes. The most visible example is the expansion of hot air. When air is heated, it expands and becomes less dense than the surrounding air, which then exerts an upward force on the hot air to, for example, make steam and smoke rise, and hot air balloons float. The same behavior happens in all liquids and gases,...
5.7K
Thermal Stress01:09

Thermal Stress

3.3K
If the temperature of an object is changed while it is prevented from expanding or contracting, the object is subjected to stress. The stress is compressive if the object expands in the absence of constraint and tensile if it contracts. This stress resulting from temperature change is known as thermal stress. It can be quite large and can cause damage. To avoid this stress, engineers may design components so they can expand and contract freely. For instance, on highways, gaps are deliberately...
3.3K
Temperature and Thermal Equilibrium01:11

Temperature and Thermal Equilibrium

9.5K
Heat and temperature are essential concepts for everyone every day. The study of heat and temperature is part of an area of physics known as thermodynamics. It is not always easy to distinguish heat and temperature.
The concept of temperature has evolved from the common concepts of hot and cold. The scientific definition of temperature explains more than just our sense of hot and cold. Temperature is operationally defined as the quantity measured with a thermometer. Furthermore, temperature is...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of personality on likelihood of contracting SARS-CoV-2 in the United States.

Personality and individual differences·2026
Same author

Expanded Tox21 Biological Assay Panel for the Prediction of Drug-Induced Liver Injury and Cardiotoxicity.

Environmental health perspectives·2026
Same author

A Hybrid Experimental and in silico Platform for ITPK1 Chemical Probe Discovery.

SLAS discovery : advancing life sciences R & D·2026
Same author

Analysis of in vitro profiling data of cosmetic ingredients within the Tox21 10K compound library for bioactivity and potential toxicity.

BMC pharmacology & toxicology·2026
Same author

Scalable hypothalamic neuron differentiation from human pluripotent stem cells suitable for modeling metabolic disorders.

Stem cell reports·2026
Same author

Early-stage drug discovery in a new-generation ultrahigh-throughput mass spectrometry platform.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 8, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.4K

A widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase.

Natalia J Martinez1, Rosita R Asawa1, Matthew G Cyr1

  • 1National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland, 20850, USA.

Scientific Reports
|June 23, 2018
PubMed
Summary

A new Split Nano Luciferase Cellular Thermal Shift Assay (SplitLuc CETSA) platform enables high-throughput drug target engagement studies in intact cells. This standardized assay accelerates pre-clinical drug discovery for diverse targets, even when other methods fail.

More Related Videos

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.3K
Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis
09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

3.0K

Related Experiment Videos

Last Updated: Feb 8, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.4K
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.3K
Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis
09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

3.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Assessing drug-target interactions in cells is crucial for pre-clinical drug discovery.
  • The Cellular Thermal Shift Assay (CETSA) measures protein thermal stability changes upon ligand binding.
  • Existing methods face challenges in throughput and applicability.

Purpose of the Study:

  • To develop and validate a high-throughput, standardized, and target-independent CETSA platform.
  • To utilize a split Nano Luciferase approach (SplitLuc CETSA) for enhanced cellular thermal shift assays.
  • To demonstrate the platform's utility for drug target engagement studies and high-throughput screening.

Main Methods:

  • Development of a homogeneous, high-throughput (384- and 1536-well) SplitLuc CETSA.
  • Validation using diverse protein targets and well-characterized inhibitors.
  • Comparison with independent biochemical and cell-based assays.

Main Results:

  • Demonstrated broad applicability of SplitLuc CETSA across various targets.
  • Showcased comparable performance to existing biochemical and cell-based assays.
  • Validated the platform for medium and high-throughput screening applications.

Conclusions:

  • SplitLuc CETSA offers a robust solution for cellular target engagement studies.
  • The platform facilitates rapid assay development and screening, especially for challenging targets.
  • Enables efficient pre-clinical drug discovery by overcoming limitations of traditional assays.