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Suicide vector for transposon mutagenesis in Pseudomonas solanacearum
Journal of Bacteriology
|September 1, 1985
Abstract:
A suicide vector was constructed by cloning the transfer genes of the wide-host-range (IncW group) plasmid R388 into the BamHI site of pBR325. This plasmid can deliver Tn5 into Pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.
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