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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

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Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule

Gayan Senavirathne1, Miguel A Lopez1, Ryan Messer1

  • 1Department of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA.

Analytical Biochemistry
|June 23, 2018
PubMed

Keywords:
Nuclease contaminantsOxygen-scavenging systemsPhotobleachingProtein purificationProtocatechuate 3,4 dioxygenaseSingle-molecule fluorescence

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