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Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients

Dalibel Bravo1, Ahmet Salduz1, Kristen L Shogren1

  • 1Dept. of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.

Gene
|June 23, 2018
PubMed

Insights

Secreted Frizzled Related Protein 3 (sFRP3) levels are significantly lower in osteosarcoma patients. This downregulation of sFRP3 in both blood and tumor tissues suggests its potential role in osteosarcoma diagnosis and treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a prevalent bone cancer in children and adolescents.
  • Wnt signaling pathways are recognized as crucial in osteosarcoma development.
  • Secreted Frizzled Related Protein 3 (sFRP3) acts as a Wnt antagonist.

Purpose of the Study:

  • To investigate the levels of sFRP3 in osteosarcoma patients.
  • To determine the correlation between sFRP3 levels and osteosarcoma pathogenesis.
  • To explore the diagnostic and therapeutic potential of sFRP3 in osteosarcoma.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) for serum sFRP3 quantification.
  • Analysis of tumor and adjacent normal tissues for sFRP3 expression.
  • Immunohistochemical analysis of tissue microarrays.
  • RNA sequencing of osteosarcoma cells.

Main Results:

  • Serum sFRP3 protein levels were significantly lower in osteosarcoma patients compared to controls.
  • Decreased sFRP3 expression was observed in 5 out of 9 tumor tissue samples.
  • Immunohistochemistry confirmed reduced sFRP3 levels in tumor tissues versus normal bone.
  • RNA sequencing revealed sFRP3 suppression and concurrent Wnt family member activation in osteosarcoma cells.

Conclusions:

  • Systemic and local levels of sFRP3 protein are downregulated in osteosarcoma.
  • sFRP3 downregulation is associated with Wnt signaling pathway alterations in osteosarcoma.
  • sFRP3 represents a potential biomarker for osteosarcoma diagnosis and patient management.
Keywords:
FrzbOsteosarcomaWntsFRP3

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