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Updated: Feb 8, 2026

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Published on: November 16, 2016
Transcriptomic study on persistence and survival of Listeria monocytogenes following lethal treatment with nisin
Shuyan Wu1, Pak-Lam Yu2, Dave Wheeler3
1Massey Institute of Food Science and Technology, Massey University, Riddet Road, 4442 Palmerston North, New Zealand.
Objectives:
The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment.
Methods:
RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).
Results:
Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.
Conclusions:
This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.
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