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Updated: Feb 8, 2026

Acute Myocardial Infarction in Rats
Published on: February 16, 2011
RNA-seq based transcriptome analysis of the protective effect of compound longmaining decoction on acute myocardial
Changli Wang1, Xihui Bai1, Shiyu Liu1
1College of Pharmacy, Shaanxi University of Chinese Medicine, Xi'an, Shaanxi, 712046, China.
Abstract:
In this study, RNA-seq technology was used to study the protective effect of Compound Longmaining (CLMN) decoction on acute myocardial infarction (AMI). The results of RNA-seq showed that the CLMN decoction has a regulatory effect on the 51 differentially expressed genes (DEGs), which were mainly enriched in the 7 pathways revealed by KEGG analysis. In addition, qPCR technology was used to verify the expression of chemokine (C-C motif) ligand 6 (Ccl6), chemokine (C-C motif) receptor 5 (Ccr5), integrin alpha M (Itgam), neutrophil cytosolic factor 1 (Ncf1), and matrix metallopeptidase 9 (Mmp9). Experiment data showed that the qPCR results were consistent with the RNA-seq results. This study demonstrated that CLMN decoction might regulate the expressions of Ccl6, Ccr5, Itgam, Ncf1 and Mmp9, inhibit the chemokine signaling pathway and leukocyte transendothelial migration to play a protective effect on AMI.
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