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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

Published on: February 9, 2011

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Evaluation of apramycin activity against methicillin-resistant, methicillin-sensitive, and vancomycin-intermediate

Katherine A Truelson1, Thea Brennan-Krohn2, Kenneth P Smith2

  • 1Boston University, Boston, MA, USA.

Diagnostic Microbiology and Infectious Disease
|June 24, 2018
PubMed

Insights

Apramycin shows promising in vitro activity against Staphylococcus aureus, including resistant strains. Further research into apramycin for human infections is recommended due to its effectiveness and rarity of resistance.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Staphylococcus aureus is a significant human pathogen.
  • Antibiotic resistance in S. aureus, including methicillin-resistant S. aureus (MRSA), poses a major public health threat.
  • Novel therapeutic strategies are needed to combat resistant bacterial infections.

Purpose of the Study:

  • To evaluate the in vitro antibacterial activity of apramycin against clinical isolates of vancomycin-intermediate and methicillin-resistant/susceptible Staphylococcus aureus.
  • To determine the minimum inhibitory concentrations (MICs) of apramycin for these S. aureus strains.
  • To assess the prevalence of apramycin resistance in the studied S. aureus population.

Main Methods:

  • In vitro susceptibility testing of clinical S. aureus strains against apramycin.
  • Determination of MIC50 and MIC90 values.
  • Comparison of obtained MICs with epidemiological cutoff (ECOFF) values.

Main Results:

  • Apramycin exhibited potent in vitro activity against Staphylococcus aureus, with MIC50/MIC90 values of 8/16 μg/mL.
  • No tested strains displayed an apramycin MIC exceeding the epidemiological cutoff value of 32 μg/mL.
  • These findings suggest that mechanisms conferring resistance to apramycin are uncommon in this clinical S. aureus population.

Conclusions:

  • Apramycin demonstrates broad-spectrum in vitro activity against diverse Staphylococcus aureus strains, including MRSA and vancomycin-intermediate strains.
  • The low frequency of resistance suggests apramycin's potential as a therapeutic agent.
  • Further investigation into apramycin and its derivatives for repurposing as human therapeutics is warranted.
Keywords:
Activity spectrumAminoglycosideAntibioticApramycinGentamicinNatural productRepurposingStaphylococcus aureusSynergy

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