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Temperature dependent Raman and X-ray diffraction studies of anhydrous milk fat.

A Lambert1, F Bougrioua1, O Abbas2

  • 1Laboratoire de Physique des Systèmes Complexes, Université Picardie Jules Verne, 33 rue S(t)Leu, 80039 Amiens cedex, France.

Food Chemistry
|June 24, 2018
PubMed
Summary

Raman spectroscopy identified distinct crystalline forms of anhydrous milk fat by analyzing vibrational modes. Temperature-dependent changes in these modes reveal structural order-disorder transitions in milk fat polymorphs.

Keywords:
Anhydrous milk fatsCrystallizationDSCLiquid statePolymorphismRaman spectroscopyX-ray diffraction

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Area of Science:

  • Food science
  • Materials science
  • Spectroscopy

Background:

  • Anhydrous milk fat (AMF) exhibits complex polymorphism crucial for its physical properties.
  • Understanding AMF's thermal behavior and structural transitions is vital for food processing and storage.

Purpose of the Study:

  • To characterize the polymorphs and liquid state of anhydrous milk fat using Raman spectroscopy.
  • To investigate the thermal evolution of specific vibrational modes in AMF.
  • To correlate spectroscopic data with structural changes and phase transitions.

Main Methods:

  • Raman spectroscopy was employed to analyze vibrational modes, focusing on ester carbonyl (1800-1700 cm⁻¹) and CH stretching (3000-2700 cm⁻¹) bands.
  • Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) were used for complementary structural analysis.
  • Temperature-dependent Raman measurements were performed to study thermal transitions.

Main Results:

  • Distinct Raman signatures were observed in the crystalline phase, indicating the coexistence of two triglyceride groups.
  • Temperature-dependent analysis of the ester carbonyl band allowed for polymorph discrimination based on mode changes and intensity ratios.
  • Increased CH stretching signals correlated with enhanced polymorph stability and crystal lattice order.
  • The order-disorder transition in AMF was successfully probed by monitoring the intensity changes of CH stretching bands with temperature.

Conclusions:

  • Raman spectroscopy is effective in characterizing AMF polymorphs and their thermal behavior.
  • Specific vibrational modes, particularly CH stretching, serve as indicators of structural order and phase transitions in AMF.
  • The study provides insights into the molecular basis of AMF polymorphism and its temperature-dependent transformations.