Related Experiment Video
Updated: Feb 8, 2026
09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
14.4K
Transcriptome analysis for identifying possible gene regulations during maize root emergence and formation at the
Sun-Goo Hwang1, Kyung-Hee Kim2, Byung-Moo Lee3
1Plant Genomics Laboratory, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Genes & Genomics
|June 24, 2018
Summary
This study identified root-specific genes crucial for early maize development using network-based transcriptome analysis. These findings reveal key gene expression patterns essential for root growth and function.
Area of Science:
- Plant Biology
- Genomics
- Molecular Biology
Background:
- Plant roots are vital for water/nutrient absorption, storage, and overall plant health.
- Understanding root development is crucial for agricultural productivity.
Purpose of the Study:
- To identify root-specific genes during early maize development using network-based transcriptome analysis.
- To elucidate the regulatory mechanisms governing initial root growth.
Main Methods:
- RNA sequencing (RNA-seq) of maize shoots and roots at different developmental stages (VE, V2).
- Bioinformatic analyses including FASTQC, TRIMMOMATIC, PageMan, MapMan, ARACNE, and Cytoscape.
- Differential gene expression analysis and co-expression network construction.
Main Results:
- Identified 1286 differentially expressed genes (DEGs) between shoots and roots.
- Detected altered mRNA levels in metabolic pathways and identified 113 transcription factors.
- Discovered two co-expressed gene modules, with one linked to maize root tip development.
Conclusions:
- Network-based transcriptome analysis successfully identified root-specific genes and regulatory elements.
- Specific gene modules are associated with the initial developmental stages of maize roots.
- The study provides insights into the genetic basis of early root development.
Related Concept Videos
03:02Primary and Secondary Growth in Roots and Shoots
60.6K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.6K
01:17Gene Regulation During Sporulation
516
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
516
02:19Circadian Rhythms and Gene Regulation
4.6K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.6K
01:27Constitutive and Regulated Gene Expression
1.2K
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1.2K
02:33Initiation of Translation
39.1K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
39.1K
01:27Emerging Adulthood
715
Jeffrey Arnett's concept of emerging adulthood offers a framework to understand the unique developmental stage between adolescence and full-fledged adulthood, generally from ages 18 to 25. This period is marked by extensive exploration and shifts in identity, relationships, and career choices, a process known in psychology as role experimentation. Emerging adulthood reflects the evolving cultural expectations surrounding adulthood and the dynamic process of personal transformation during...
715