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Updated: Feb 8, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Paper-based electrochemical sensor for on-site detection of the sulphur mustard
Noemi Colozza1, Kai Kehe2, Tanja Popp3,4
1Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome, Italy.
Abstract:
Herein, we report a novel paper-based electrochemical sensor for on-site detection of sulphur mustards. This sensor was conceived combining office paper-based electrochemical sensor with choline oxidase enzyme to deliver a sustainable sensing tool. The mustard agent detection relies on the evaluation of inhibition degree of choline oxidase, which is reversibly inhibited by sulphur mustards, by measuring the enzymatic by-product H2O2 in chronoamperometric mode. A nanocomposite constituted of Prussian Blue nanoparticles and Carbon Black was used as working electrode modifier to improve the electroanalytical performances. This bioassay was successfully applied for the measurement of a sulphur mustard, Yprite, obtaining a detection limit in the millimolar range (LOD = 0.9 mM). The developed sensor, combined with a portable and easy-to-use instrumentation, can be applied for a fast and cost-effective detection of sulphur mustards.
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