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Related Experiment Video

Updated: Feb 8, 2026

Photodeposition of Pd onto Colloidal Au Nanorods by Surface Plasmon Excitation

Photodeposition of Pd onto Colloidal Au Nanorods by Surface Plasmon Excitation

Published on: August 15, 2019

Surface plasmon-assisted microscope.

Julian Borejdo^1,2, Zygmunt Gryczynski³, Rafal Fudala^1,2

¹University of North Texas, Health Science Center, Department of Microbiology, Immunology and Genetic, United States.

Journal of Biomedical Optics

|June 24, 2018

Summary

This summary is machine-generated.

This study introduces a novel microscopy technique using gold films and surface plasmons to image cell membranes with enhanced resolution. This method offers a powerful alternative to Total Internal Reflection Fluorescence microscopy for studying molecular dynamics near cell surfaces.

Keywords:

fluorescence microscopy surface plasmon coupled emission

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Last Updated: Feb 8, 2026

Photodeposition of Pd onto Colloidal Au Nanorods by Surface Plasmon Excitation

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Published on: August 15, 2019

Engineering Antiviral Agents via Surface Plasmon Resonance

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Published on: June 14, 2022

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

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Published on: June 23, 2022

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Total Internal Reflection Fluorescence (TIRF) microscopy images thin cellular layers near coverslips.
Existing TIRF methods have limitations in resolution and complexity.

Purpose of the Study:

To develop an alternative imaging method for proximal-to-basal cell membranes.
To enhance imaging resolution and simplify the optical setup compared to TIRF.

Main Methods:

Utilized a high refractive index coverslip coated with a thin gold layer.
Employed Kretschmann illumination and surface plasmon resonance.
Collected fluorescence via a high numerical aperture objective and EMCCD or avalanche photodiode.

Main Results:

Achieved imaging of thin-layer proximal-to-basal membranes.
Reduced detection layer thickness below 10 nm via metal quenching.
Demonstrated excellent background rejection and avoidance of TIRF complications.

Conclusions:

The novel method provides a powerful tool for studying molecular motion near cell surfaces.
This technique offers advantages over TIRF in terms of resolution and optical setup simplicity.