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Urinary l-kynurenine quantification and selective extraction through a molecularly imprinted solid-phase extraction

Lucia Mergola1, Ciriana Orabona2, Elisa Albini2

  • 1Department of Engineering for Innovation, University of Salento, Lecce, Italy.

Journal of Separation Science
|June 24, 2018
PubMed
Summary

A novel molecularly imprinted polymer enables specific extraction of l-kynurenine, a potential biomarker for antitumor therapy efficacy. This method allows accurate quantification of l-kynurenine in human urine, aiding drug development.

Keywords:
biomarkershuman urinel-kynureninemolecularly imprinted polymerssolid-phase extraction

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • l-Kynurenine is an endogenous metabolite from l-tryptophan catabolism.
  • It shows potential as a biomarker for evaluating checkpoint inhibitor efficacy in antitumor therapy.
  • l-Kynurenine is also explored for its therapeutic potential in autoimmune disorders.

Purpose of the Study:

  • To develop a specific molecularly imprinted polymer for l-kynurenine recognition.
  • To establish an innovative solid-phase extraction (SPE) method for l-kynurenine quantification in human urine.
  • To assess the feasibility of the developed method for pharmaceutical applications.

Main Methods:

  • Synthesis of a molecularly imprinted polymer tailored for l-kynurenine.
  • Application of the polymer in an off-line solid-phase extraction technique.
  • Validation of the method using standard solutions and fortified human urine samples.

Main Results:

  • High recoveries achieved: up to 97.7% for standards and 84.1% for urine samples.
  • Excellent linearity demonstrated in the range of 0.157–20 μg/mL (r² = 0.9992).
  • Successful quantification of low l-kynurenine concentrations (0.80 μg/mL) in human urine.

Conclusions:

  • The developed SPE method using a specific molecularly imprinted polymer is effective for l-kynurenine extraction and quantification in human urine.
  • This method shows good feasibility for biological samples and aids in identifying potential drug candidates.
  • The technology holds promise for pharmaceutical industries in l-kynurenine production for autoimmune disorder treatments.